Monitoring Assays for Mtbe Toxicity and Genotoxicity
نویسندگان
چکیده
This research is focused on determination of toxicity, genotoxicity and discovery of MTBE-degrading bacterial strains. Toxicity was detected using bacterial luminescent method Microtox while genotoxicity was monitored by Ames test with Salmonella typhimurium his (TA 98, TA 100, YG 1041 and YG 1042) as indicator strains. Potential MTBE-degrading strains were tested by Bioscreen. This method monitors the growth of bacterial strains in the presence of MTBE as the sole source of carbon and under different nutritional limits. MTBE is utilise via cometabolim of organic substrates (alkanes, acetone, cyclohexane, toluene or lactate), respectively. Two strains of Pseudomonas spp. and soil isolates were employed for measurement of specific growth rates μ. Oxygen consumption of the tested strains was measured by OxiTop.
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